fluorescein vector laboratories (Vector Laboratories)

Vector Laboratories is a verified supplier

Vector Laboratories manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Vector Laboratories fluorescein vector laboratories
Fluorescein Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/FL-1351-2/Fluorescein+labeled+Wisteria+Floribunda+Lectin+(WFA%2C+WFL)/pm41996240-332-35-36
Average 95 stars, based on 147 article reviews

fluorescein vector laboratories - by Bioz Stars, 2026-11

95/100 stars

Images

Incubation:

Article Title: Prenatal stress history modifies adolescent stress effects on adult social behavior and basolateral amygdala GABAergic neurons with perineuronal nets.
Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

Article Title: Neurobehavioral Effects of “Dry Hit” Nicotine E-Cigarette Vapor Inhalation in Adolescent Wistar Rats
Article Snippet: .. 0.3% Triton-X, 2% goat serum, 1% BSA was used as a blocking agent; tissue slices were incubated in primary antibodies Wisteria Floribunda Lectin Antibody, Fluorescein (FL-1351-2; Vector; 1:500), Anti-NeuN Antibody, clone A60MAB377 (Sigma-Aldrich; 1:1000), and Parvalbumin Polyclonal Antibody, Unconjugated, Host: Rabbit / IgG (PA1-933; Invitrogen; 1:10,000) overnight. .. The second day of IHC involved incubating the slices in the secondary antibodies (NeuN: Goat anti-mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568, 1:200; PV: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647, 1:1000) and then mounting the slices on microscope slides (FisherScientific).

Article Title: Effects of Complete Spinal Cord Transection in Neonatal and Juvenile Rats on Parvalbumin-Positive Inputs and Perineuronal Net Formation on Chx10-Positive V2a Interneurons
Article Snippet: A series of sections was incubated in a moist chamber overnight at 4°C with a mixture of (1) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and rabbit polyclonal IgG antibody against parvalbumin (1:200, Synaptic Systems, Göttingen, Germany), (2) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc.) and mouse monoclonal IgM antibody against CSA (1:200, NU-07-001, clone 2H6, Cosmo Bio Co., Ltd, Tokyo, Japan), or (3) mouse monoclonal IgG antibody against Chx10 (1:200, D-11, Santa Cruz Biotechnology, Inc.) only, diluted with 1% normal donkey serum, 0.2% bovine serum albumin, and 0.1% NaN 3 in 0.025 M phosphate-buffered saline with Tween 20 (PBST). .. The sections were rinsed several times with 0.025 M PBST and then incubated for 2 h at room temperature with a mixture of secondary antibodies: (1) Alexa Fluor 488-conjugated donkey anti-rabbit IgG (10 μg/mL; Jackson ImmunoResearch Laboratories, West Grove, PA, USA), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL; Jackson ImmunoResearch Laboratories), and NeuroTrace 640/660 (1:100, N21483 , Life Technologies Corporation, OR, USA); (2) Alexa Fluor 488-conjugated donkey anti-mouse IgM (10 μg/mL; Jackson ImmunoResearch Laboratories), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL), and NeuroTrace 640/660 (1:100); or (3) fluorescein-conjugated WFA (1:100, FL-1351, Vector Laboratories, Inc., Burlingame, CA, USA), cyanine Cy3-conjugated donkey anti-mouse IgG (10 μg/mL), and NeuroTrace 640/660 (1:100), diluted with 1% normal donkey serum, 0.2% bovine serum albumin, and 0.1% NaN 3 in 0.025 M PBST. .. The antibody specificity was verified by incubation with 0.5% normal mouse serum (Jackson ImmunoResearch Laboratories) or 0.5% normal rat serum (Jackson ImmunoResearch Laboratories) instead of the primary antibodies.

Article Title: Amygdala stress-responsive ensembles mediate distinct susceptibility to negative stress-induced remote depression in adolescent and adult mice.
Article Snippet: .. The brain slices were transferred into 10% donkey serum with a primary antibody (Anti-c-Fos, 1:1000, SYSY; Anti-CaMKIIα, 1:400, CST; Anti-GABA, 1:400, Invitrogen; Anti-Parvalbumin Antibody, Novus, 1:1000; Wisteria Floribunda Lectin (WFA, WFL), Fluorescein (FL-1351), Vector Laboratories, 1:400; Anti-Iba1, 1:400, HUABIO; Anti-Iba1, 1:400, Abcam; Anti-CD68, 1:400, BIORAD; AntiPSD95, 1:200, Bioss Antibodies; Anti-vGLUT1, 1:500, SYSY; Anti-GAD65/67, 1:100, Abcam; Anti-Gephyrin, 1:400, Santa Cruz Biotechnology) and incubated at 4◦C for 48 h. After rinsing with PBS for 10 min × 3 times, the brain slices were transferred into 5% donkey serum with a secondary antibody (Alexa Fluor 488, 1:400, Invitrogen; Alexa Fluor 594, 1:400, Invitrogen; Alexa Fluor 555, 1:400, Invitrogen; Alexa Fluor 647, 1:400, Invitrogen) and incubated in the dark at room temperature for 2 h. The patches were rinsed three times with PBS 10 min × 3 times, and then dried with a 4′, 6-diamidino-2-phenylindole staining solution for immunofluorescence staining. .. Images were obtained using a confocal laser microscope (Leica STELLARIS 5, Germany).

Staining:

Article Title: Glial cell and perineuronal net interactions in the dorsal striatum of aged mice
Article Snippet: .. Coronal 30-μm hemisections containing the dorsal striatum were processed in two independent staining/imaging cohorts: ( ) microglia were labeled with Iba1 (rabbit; FUJIFILM Wako, #P3088) in combination with the lysosomal/phagocytic marker CD68 (rat; Bio-Rad, #MCA1957) and perineuronal nets (PNNs) were visualized using fluorescein-conjugated Wisteria floribunda agglutinin (WFA; Vector Laboratories, #FL-1351); ( ) astrocytes were labeled with GFAP (rabbit; Cell Signaling Technology, #12389) together with WFA to visualize PNNs. .. Secondary antibodies included goat anti-rat Alexa Fluor 594 (Invitrogen, #A48264), goat anti-rabbit Alexa Fluor 635 (Invitrogen, #A31577), and goat anti-mouse Alexa Fluor 594 (Invitrogen, #A21125), as appropriate for each experiment.

Article Title: Amygdala stress-responsive ensembles mediate distinct susceptibility to negative stress-induced remote depression in adolescent and adult mice.
Article Snippet: .. The brain slices were transferred into 10% donkey serum with a primary antibody (Anti-c-Fos, 1:1000, SYSY; Anti-CaMKIIα, 1:400, CST; Anti-GABA, 1:400, Invitrogen; Anti-Parvalbumin Antibody, Novus, 1:1000; Wisteria Floribunda Lectin (WFA, WFL), Fluorescein (FL-1351), Vector Laboratories, 1:400; Anti-Iba1, 1:400, HUABIO; Anti-Iba1, 1:400, Abcam; Anti-CD68, 1:400, BIORAD; AntiPSD95, 1:200, Bioss Antibodies; Anti-vGLUT1, 1:500, SYSY; Anti-GAD65/67, 1:100, Abcam; Anti-Gephyrin, 1:400, Santa Cruz Biotechnology) and incubated at 4◦C for 48 h. After rinsing with PBS for 10 min × 3 times, the brain slices were transferred into 5% donkey serum with a secondary antibody (Alexa Fluor 488, 1:400, Invitrogen; Alexa Fluor 594, 1:400, Invitrogen; Alexa Fluor 555, 1:400, Invitrogen; Alexa Fluor 647, 1:400, Invitrogen) and incubated in the dark at room temperature for 2 h. The patches were rinsed three times with PBS 10 min × 3 times, and then dried with a 4′, 6-diamidino-2-phenylindole staining solution for immunofluorescence staining. .. Images were obtained using a confocal laser microscope (Leica STELLARIS 5, Germany).

Labeling:

Article Title: Ventral pallidal perineuronal nets regulate opioid relapse
Article Snippet: In brief, sections were washed in PBS on a shaker, blocked using a glycoprotein-free blocking solution (Vector Laboratories; SP-5040-125) containing 0.25% Triton X-100 for 30 minutes at room temperature, incubated in 0.25% PBS-Triton (PBS-T) containing primary antibodies overnight at 4°C, and incubated in PBS-T containing secondary antibodies for 3h at room temperature. .. Following immunolabeling, perineuronal nets were visualized using Wisteria Floribunda Agglutin (WFA) conjugated to fluorescein (1:500, Vector Laboratories; FL-1351; RRID:AB_2336875) or biotin (1:200, Vector Laboratories; B-1355; RRID:AB_2336874) in PBS-T for 30 minutes at room temperature, followed by a streptavidin labeling step (in PBS-T; 1h at room temperature). ..

Marker:

Blocking Assay:

Immunolabeling:

Virus:

Article Title: Amygdala stress-responsive ensembles mediate distinct susceptibility to negative stress-induced remote depression in adolescent and adult mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-c-Fos Synaptic Systems Cat#226008; RRID:AB_2891278 Mouse anti-CaMKllα(6G9) Cell Signaling Technology Cat#50049S; RRID:AB_2721906 Rabbit anti-GABA Invitrogen Cat#PA5-32241; RRID:AB_2549714 Anti-Parvalbumin Antibody Novus Cat# NB120-11427; RRID:AB_791498 Wisteria Floribunda Lectin (WFA, WFL), Fluorescein Vector Laboratories Cat# FL-1351; RRID:AB_2336875 Rat anti-Iba1 HUABIO Cat#HA601367; RRID:AB_3070606 Goat anti-Iba1 Abcam Cat#AB5076; RRID:AB_2224402 Rat anti-CD68 BIORAD Cat#163145; RRID:AB_2885063 Gpig anti-PSD95 Bioss Antibodies Cat# bs-20649R; RRID:AB_11101933 Rabbit anti-vGLUT1 Synaptic Systems Cat#135318; RRID:AB_2924948 Rabbit anti-GAD65/67 Abcam Cat#183999; RRID:AB_3662875 Mouse anti-Gephyrin Santa Cruz Biotechnology Cat#C0220; RRID:AB_397929 Alexa fluor 488-anti-Rabbit secondary antibody Invitrogen Cat#A21206; RRID:AB_2535792 Alexa fluor 488-anti-Mouse secondary antibody Invitrogen Cat#A32766; RRID:AB_2762823 Alexa fluor 488-anti-Rat secondary antibody Invitrogen Cat#A48269 RRID:AB_2893137 Alexa fluor 488-anti-Rabbit secondary antibody Invitrogen Cat#A21207; RRID:AB_141637 Alexa fluor 488-anti-Goat secondary antibody Invitrogen Cat#2604365; RRID: AB_10055703 Alexa fluor 647-anti-Rabbit secondary antibody Invitrogen Cat#A32795; RRID:AB_2762835 Alexa fluor 594-anti-Mouse secondary antibody Invitrogen Cat# A21203 RRID:AB_2474956 Alexa fluor 555-anti-Gpig secondary antibody Abcam Cat#AB150186 RRID:AB_3099754 Bacterial and virus strains rAAV-cFos-tTA-NLS-FLAG Brain Case Cat#BC-0058 rAAV-TRE3G-mCherry Brain Case Cat#BC-0035 rAAV-TRE3G-hM3D(Gq)-mCherry Brain Case Cat#BC-0686 rAAV-TRE-tight-hM4D(Gi)-mCherry Brain Case Cat#BC-0151 rAAV-CaMKIIα-WPRE-hGH polyA Brain Case Cat#PT-0220 rAAV-EF1α-DIO-hChR2(H134R)-mCherry-WRPE-hGH pA BrainVTA Cat#PT-0002 rAAV-PV-CRE-hGH polyA BrainVTA Cat#PT-0275 rAAV-hSyn-DIO-EGFP-WPREs-hGH BrainVTA Cat#PT-1103 rAAV-hSyn-DIO-hM3D(Gq)-EGFP-WPREs BrainVTA Cat#PT-0891 rAAV-hSyn-DIO-hM4D(Gi)-EYFP-WPRE-hGH polyA BrainVTA Cat#PT-0344 Chemicals, peptides, and recombinant proteins Doxycycline (DOX) Sigma-Aldrich Cat#D9891-10G Sodium chloride Sigma-Aldrich Cat#SLBS7447 Clozapine N-oxide (CNO) APE×BIO Cat#GC10822 Bicuculline (Bic) Selleckchem Cat#S7071 Chondroitinase ABC (ChABC) Sigma-Aldrich Cat#C3667 (Continued on next page) Cell Reports 45, 117281, April 28, 2026 21 .. Male C57BL/6J mice were purchased from the Experimental Animal Center of Xuzhou Medical University.

Recombinant:

Article Title: Amygdala stress-responsive ensembles mediate distinct susceptibility to negative stress-induced remote depression in adolescent and adult mice.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit anti-c-Fos Synaptic Systems Cat#226008; RRID:AB_2891278 Mouse anti-CaMKllα(6G9) Cell Signaling Technology Cat#50049S; RRID:AB_2721906 Rabbit anti-GABA Invitrogen Cat#PA5-32241; RRID:AB_2549714 Anti-Parvalbumin Antibody Novus Cat# NB120-11427; RRID:AB_791498 Wisteria Floribunda Lectin (WFA, WFL), Fluorescein Vector Laboratories Cat# FL-1351; RRID:AB_2336875 Rat anti-Iba1 HUABIO Cat#HA601367; RRID:AB_3070606 Goat anti-Iba1 Abcam Cat#AB5076; RRID:AB_2224402 Rat anti-CD68 BIORAD Cat#163145; RRID:AB_2885063 Gpig anti-PSD95 Bioss Antibodies Cat# bs-20649R; RRID:AB_11101933 Rabbit anti-vGLUT1 Synaptic Systems Cat#135318; RRID:AB_2924948 Rabbit anti-GAD65/67 Abcam Cat#183999; RRID:AB_3662875 Mouse anti-Gephyrin Santa Cruz Biotechnology Cat#C0220; RRID:AB_397929 Alexa fluor 488-anti-Rabbit secondary antibody Invitrogen Cat#A21206; RRID:AB_2535792 Alexa fluor 488-anti-Mouse secondary antibody Invitrogen Cat#A32766; RRID:AB_2762823 Alexa fluor 488-anti-Rat secondary antibody Invitrogen Cat#A48269 RRID:AB_2893137 Alexa fluor 488-anti-Rabbit secondary antibody Invitrogen Cat#A21207; RRID:AB_141637 Alexa fluor 488-anti-Goat secondary antibody Invitrogen Cat#2604365; RRID: AB_10055703 Alexa fluor 647-anti-Rabbit secondary antibody Invitrogen Cat#A32795; RRID:AB_2762835 Alexa fluor 594-anti-Mouse secondary antibody Invitrogen Cat# A21203 RRID:AB_2474956 Alexa fluor 555-anti-Gpig secondary antibody Abcam Cat#AB150186 RRID:AB_3099754 Bacterial and virus strains rAAV-cFos-tTA-NLS-FLAG Brain Case Cat#BC-0058 rAAV-TRE3G-mCherry Brain Case Cat#BC-0035 rAAV-TRE3G-hM3D(Gq)-mCherry Brain Case Cat#BC-0686 rAAV-TRE-tight-hM4D(Gi)-mCherry Brain Case Cat#BC-0151 rAAV-CaMKIIα-WPRE-hGH polyA Brain Case Cat#PT-0220 rAAV-EF1α-DIO-hChR2(H134R)-mCherry-WRPE-hGH pA BrainVTA Cat#PT-0002 rAAV-PV-CRE-hGH polyA BrainVTA Cat#PT-0275 rAAV-hSyn-DIO-EGFP-WPREs-hGH BrainVTA Cat#PT-1103 rAAV-hSyn-DIO-hM3D(Gq)-EGFP-WPREs BrainVTA Cat#PT-0891 rAAV-hSyn-DIO-hM4D(Gi)-EYFP-WPRE-hGH polyA BrainVTA Cat#PT-0344 Chemicals, peptides, and recombinant proteins Doxycycline (DOX) Sigma-Aldrich Cat#D9891-10G Sodium chloride Sigma-Aldrich Cat#SLBS7447 Clozapine N-oxide (CNO) APE×BIO Cat#GC10822 Bicuculline (Bic) Selleckchem Cat#S7071 Chondroitinase ABC (ChABC) Sigma-Aldrich Cat#C3667 (Continued on next page) Cell Reports 45, 117281, April 28, 2026 21 .. Male C57BL/6J mice were purchased from the Experimental Animal Center of Xuzhou Medical University.

Immunofluorescence:

Article Title: Amygdala stress-responsive ensembles mediate distinct susceptibility to negative stress-induced remote depression in adolescent and adult mice.
Article Snippet: .. The brain slices were transferred into 10% donkey serum with a primary antibody (Anti-c-Fos, 1:1000, SYSY; Anti-CaMKIIα, 1:400, CST; Anti-GABA, 1:400, Invitrogen; Anti-Parvalbumin Antibody, Novus, 1:1000; Wisteria Floribunda Lectin (WFA, WFL), Fluorescein (FL-1351), Vector Laboratories, 1:400; Anti-Iba1, 1:400, HUABIO; Anti-Iba1, 1:400, Abcam; Anti-CD68, 1:400, BIORAD; AntiPSD95, 1:200, Bioss Antibodies; Anti-vGLUT1, 1:500, SYSY; Anti-GAD65/67, 1:100, Abcam; Anti-Gephyrin, 1:400, Santa Cruz Biotechnology) and incubated at 4◦C for 48 h. After rinsing with PBS for 10 min × 3 times, the brain slices were transferred into 5% donkey serum with a secondary antibody (Alexa Fluor 488, 1:400, Invitrogen; Alexa Fluor 594, 1:400, Invitrogen; Alexa Fluor 555, 1:400, Invitrogen; Alexa Fluor 647, 1:400, Invitrogen) and incubated in the dark at room temperature for 2 h. The patches were rinsed three times with PBS 10 min × 3 times, and then dried with a 4′, 6-diamidino-2-phenylindole staining solution for immunofluorescence staining. .. Images were obtained using a confocal laser microscope (Leica STELLARIS 5, Germany).

Review